syslinux boot from usb flash drive

Sure. First download the DSL bootusb.img from one of the download sites. I would first start by making the boot floppy from this .img file. Next grab your pendrive, you don’t need to format it, now copy over the KNOPPIX folder. Next test your setup by booting from the floppy. DSL will run from the USB pendrive. Now, if your machine will boot from USB, copy the contents of the floppy onto the root directory of the pendrive. Do not copy to inside the KNOPPIX folder. Then go grab syslinux from syslinux
You only need to run syslinux with a target of the usb pendrive. On windows for example do syslinux.exe -s E: Where drive E corresponds to your pendrive. Finally change your BIOS to boot from USB. I also had to change my BIOS to usb-keyboard in order for the boot from USB to work.